An autoanalyzer procedure for serum iron and total iron-binding capacity, with use of ferrozine.
نویسندگان
چکیده
An AutoAnalyzer method has been developed in which the recently introduced reagent for iron, 3. (2.pyridyl).5,6.bis(4.phenylsulfonic acid).1,2,4.triazine (“ferrozine”), is used. With the Technicon colorimeter or Gilford 300-N spectrophotometer in use, sampling rates may be 30/h or 50/h, respectively. Sample volume is 0.8 ml, and measurements are made at 560 nm. A procedure for measuring total iron-binding capacity is also described.
منابع مشابه
Effects of Alpha-Lipoid Acid on Serum Iron, Total Iron Binding Capacity and Hematological Indices in β-Thalassemia Major Patients: A Cross Over Clinical Trial
Background and Objectives: Accumulation of iron is the most common consequences of repeated blood transfusions in β-thalassemia major patients. Iron chelating effects of alpha-lipoic acid have been reported by several studies. The aim of this study was to investigate effects of supplementation with alpha-lipoic acid, as a chelator, on serum iron and hematological indices in these patients. Ma...
متن کاملAn Accurate Spectrophotom etric Method for Serum Iron and Iron-B inding Capacity without Deproteinization or C entrifugation*!
A simple, direct semi-micro method has been developed for serum iron and latent iron-binding capacity. For total serum iron, into a one cm cuvet are pipetted (a ) 1.5 ml of Na acetate buffer (pH 4.5) containing Na ascorbate and (b ) 0.3 ml of serum. The serum blank absorbance is measured at 562 nm, and 0.2 ml of ferrozine color reagent is added. After incubation (42°, 20 min), the absorbance is...
متن کاملFerrozine iron and total iron-binding capacity method adapted to the ABA-100 Bichromatic Analyzer.
A sensitive method (Clin. Chem. 26: 327--331, 1980) for serum iron, in which the color reagent Ferrozine is used, is modified and adapted to the Abbott ABA-100 discrete analyzer. The standard curve is linear to at least 10 mg/L and the method showed day-to-day precision (CV) of 2.4% for a 1.03 mg/L sample (n = 63) and 1.9% for a 2.13 mg/L sample (n = 63). Lower values were obtained than with th...
متن کاملInvestigating Changes in the Serum Iron, TIBC (Total Iron Binding Capacity) and Transferrin Levels in Patients with Morbid Obesity Undergoing Bariatric Surgery in Kerman in 2018: A Short Report
Background and Objectives: Bariatric surgery is one of the most effective treatments for obesity. This study was designed to investigate changes in the serum iron, TIBC (Total Iron Binding Capacity) and transferrin levels in patients with morbid obesity undergoing bariatric surgery in Kerman in 2018. Materials and Methods: This prospective study was carried out on 65 participants who had gone ...
متن کاملThe measurement of iron-binding capacity in serum and purified transferrin with the aid of chemical affinity chromatography.
In a mew method for the estimation of transferrin by iron-binding capacity iron is added as the tartrate in NaCl with about 10 mM bicarbonate. The excess iron is removed by passage through DEAE-Sephadex A-50 previously treated with the iron chelator disodium catechol-3,5-disulphonate. The iron remaining bound to transferrin is measured without protein precipitation by the use of ferrozine. The ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 17 9 شماره
صفحات -
تاریخ انتشار 1971